The Nephroprotective Effect of Zizphus Jujuba Extract Against 5-Flurouracil- Induced Nephropathy

Aiman A. Shoiab1, Ahmed R. Gardouh1,2*

        1Faculty of Pharmacy, Department of Pharmacy Practice, Jadara University, 21110 Irbid, Jordan.

2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, 41522 Ismailia, Egypt.

* Corresponding author:2Ahmed.ga@jadar.edu.jo

DOI: https://doi.org/10.32441/kjps.04.02.p6

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ABSTRACT:

This study assessed the protective effect of Zizphus jujube (ZJ) extract on 5‑FU‑induced alterations in renal function markers and kidney morphology in Dawley rats. Twenty-four rats were divided randomly into four groups administrated orally with 0.9% normal saline as the control group, 5-FU (40 mg/kg daily for 5 days), ZJ (500 mg/ kg daily for 5 days), and 5-FU‏+ ZJ (for 6 days). further biochemical experiments carried out on blood collected from the heart. Kidney tissues were obtained for analysis of catalase (Cat), glutathione S-transfers (GST), and lipid peroxide levels as well as histology analysis. 5-FU significantly reduced the enzyme activity of Cat and GST and increased levels of lipid peroxidation and plasma creatinine levels (P < 0.005). Histopathological examination showed severe wide ischemia of proximal convoluted tubule (PCT), missing in Bowman’s space, and edema in the group treated with 5-FU. In addition, pretreatment with ZJ has significantly improved levels of Cat and GST and reduced lipid peroxidation and plasma creatinine levels (P < 0.05). Moreover, the histopathological analysis showed that ZJ relatively prevented the damage in renal tubular cells compared with 5-FU treated group. Supplementation with ZJ may have clinical benefit in nephrotoxicity caused by 5‑FU.

Keywords: antioxidant; 5-Flurouracil, ziziphus jujube, nephrotoxicity, glutathione S-transferase, catalase, histological analysis.